Work

Deciphering allosterism of an Escherichia coli hexuronate metabolism regulator: UxuR

Year: 2025

Type: article

Abstract: . The identified residues can be divided into those that are critical for maintaining protein stability and those that are essential for facilitating allosteric communication from the effector domain to the DNA-binding site. A particularly intriguing discovery was the identification of a variant that could respond to different sugars, enriching our understanding of hexuronate metabolism flexibility. The identification of UxuR variants with altered ligand specificity not only advances our understanding of bacterial metabolism regulation but also opens new avenues for developing antimicrobial strategies targeting hexuronate pathways.

Source: RSC Medicinal Chemistry

DOI: 10.1039/d5md00391a

Authors: Beatriz C. Almeida, Sean A. Wirt, Kristala L. J. Prather, Alexandra T. P. Carvalho

Institutions: Centro de Neurociências e Biologia Celular, Centro de Inovação em Biomedicina e Biotecnologia, University of Coimbra, Massachusetts Institute of Technology, Almac (United Kingdom)

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Fulbright Portugal, Fundação para a Ciência e a Tecnologia

Awards: G1672043847, Role of metal ions in DNA recognition by proteins, The role of Zinc in transcription factor allosteric regulation, G7082142669, G7991599336