Work

Light-controlled genome editing by activation of Cas9-mRNA translation

Year: 2025

Type: article

Abstract: transcription. This is the first demonstration of using light to activate muted Cas9-mRNA, leading to permanent alterations on the DNA level, despite the messenger itself being transient in nature.

Source: Chemical Science

DOI: 10.1039/d5sc01999k

Authors: Helena Schepers, Greta Charlotte Dahm, Martin Sumser, Sabine Hüwel, Andrea Rentmeister

Institutions: University of Münster, Ludwig-Maximilians-Universität München

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: European Commission, Ludwig-Maximilians-Universität München, H2020 European Research Council

Awards: G434337789, Optochemical control of cell fate by activation of mRNA translation

OpenAlex ID: W4411837425

FWCI: 1.567

Cites: 19

Cited by: 6

Related to: 10