Work

SEMA3B switches axon-axon to axon-glia interactions required for unmyelinated axon envelopment and integrity

Year: 2025

Type: article

Abstract: During peripheral nerve (PN) development, unmyelinated axons (nmAs) tightly fasciculate before being separated and enveloped by non-myelinating Schwann cells (nmSCs), glial cells essential for maintaining nmA integrity. How such a switch from axon-axon to axon-glia interactions is achieved remains poorly understood. Here, we find that inactivating SC-derived SEMA3B or its axonal receptor components in mice leads to incomplete nmA separation and envelopment by nmSCs, eliciting hyperalgesia and allodynia. Conversely, increasing SEMA3B levels in SCs accelerates nmA separation and envelopment. SEMA3B transiently promotes nmA defasciculation accompanied by cell adhesion molecule (CAM) endocytosis, subsequently facilitating nmA-nmSC association. Restoring SEMA3B expression following PN injury promotes nmA-nmSC re-association and alleviates hyperalgesia and allodynia. We propose that SEMA3B-induced CAM turnover facilitates a switch from axon-axon to axon-glia interactions promoting nmA envelopment by nmSCs, which may be exploitable for alleviating PN injury-induced pain by accelerating the restoration of nmA integrity. Nerve fibers relaying pain information from peripheral organs are normally supported by specialized glial cells called nonmyelinating Schwann cells. Here, the authors show that a secreted factor, SEMA3B, promotes nerve fiber-Schwann cell interactions necessary for suppressing pathological pain.

Source: Nature Communications

DOI: 10.1038/s41467-025-61318-8