Year: 2025
Type: article
Abstract: ABSTRACT Flavin cofactors are attractive Electron Spin Resonance (ESR) probes for proteins because cellular reductants and light can generate their semiquinone states. We have used ESR spectroscopy to study the bacterial transmembrane aerotaxis receptor (Aer) in its native Escherichia coli membrane environment. Optimization of the spectroscopic (electronic relaxation times) and cell growth (isotopic labeling) conditions allowed for measurements of Aer with its partners - the histidine kinase (CheA) and the coupling protein (CheW) - in native signaling arrays. Continuous-wave ESR measurements at room temperature showed a rigid Aer flavin immobilized in the cofactor pocket and Q-band electron nuclear double resonance (ENDOR) measurements identified a predominant anionic semiquinone radical state in cell . Q-band four-pulse double electron-electron resonance (4P-DEER) measurements indicated a 4.1 nm distance between the two flavins of an Aer homodimer, consistent with previous in vitro measurements, but also revealed additional separations in cell indicative of chemoreceptor arrays, not previously observed for Aer. For general application, we further developed a genetically encoded Light-Oxygen and Voltage (LOV) domain for incorporation into target proteins as an ESR probe of structural properties in cell . This approach provides a framework to elucidate protein oligomeric states and conformations that are difficult to reproduce in vitro .
Source: Nature Communications
Institution: Cornell University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: Advancing our knowledge of viral membrane fusion and of IDP-membrane interactions by ESR, G3700135084, G403320550, Molecular mechanisms of signaling systems responsive to light, redox and chemical environment, G608552809, G6639989534, G6887104853, National Biomedical Resource for Electron-Spin Resonance Spectroscopy (ACERT), G7512093943, G757445101, G7742344631, G8259810505, National Biomedical Center for Advanced ESR Technology (ACERT), G9000173621, G9000273646
OpenAlex ID: W4411900525