Supplementary Figure S13 from Single-cell Transcriptional Atlas of Human Hematopoiesis Reveals Genetic and Hierarchy-Based Determinants of Aberrant AML Differentiation
Year: 2025
Type: supplementary-materials
Abstract: Supplementary Figure S13. Clinical relevance of lymphoid and erythroid lineage biases A) BoneMarrowMap projections of representative AML patient samples with mono-allelic mutations in the RUNX1 gene. B-D) Erythroid vs myeloid involvement within 136 AEL samples from Iacobucci et al 2019 (72), depicted as the difference between the inferred Early Erythroid abundance and GMP abundance in each sample. These are depicted in association with C) driver mutation status and D) clinical risk category as defined in the original study. P values from a two-tailed Wilcoxon rank-sum test are shown. Box plots indicate the range of the central 50% of the data with the central line marking the median. Whiskers extend from each both to 1.5x the interquartile range. E-F) Principal component analysis of AML differentiation states within 357 normal karyotype adult AML samples. Heatmap columns in (F) represent AML samples ordered by principal component 2 (PC2), representing the Lymphoid vs Erythroid axis. For each AML sample, mutation status as well as estimated relative abundance of each AML differentiation state are shown. To the left of the heatmap, a bar plot with the feature loadings for PC2 is shown, representing the specific coefficients assigned to each AML differentiation state for calculation of PC2. G) Volcano plot of Pearson correlations between the relative abundance of each AML differentiation state and ex vivo drug sensitivities (area under the dose response curve x -1) from the BeatAML2 (31) screen for 123 normal karyotype AML samples. Associations of differentiation states with higher drug sensitivities are colored in red and associations with lower drug sensitivities are colored in blue.
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4411907271