Antimicrobial efficacy of mangosteen, chitosan, and their combination as intracanal medicaments against Enterococcus faecalis: A randomized control clinical trial
Year: 2025
Type: article
Study designs: Randomized Controlled Trial, Clinical Trial
Abstract: Context: The increasing prevalence of antibiotic resistance and concerns regarding adverse drug reactions have prompted interest in natural alternatives for antimicrobial treatments, particularly in endodontics. Aims: This study aimed to evaluate the antimicrobial effectiveness of mangosteen, chitosan, and their combination as intracanal medicaments against Enterococcus faecalis . Settings and Design: A double-blind, randomized clinical trial was conducted involving 40 patients with single-rooted nonvital teeth ( n = 10 per group). Patients were assigned to four groups: Group 1 – Mangosteen, Group 2 – Chitosan, Group 3 – Mangosteen with Chitosan, and Group 4 – Calcium Hydroxide (Ca(OH) 2 , control). Samples were collected before chemo-mechanical preparation (S1) and after 7 days of medicament application (S2). Subjects and Methods: Following standard intracanal procedures, medicaments were applied, and bacterial samples were collected at baseline and after treatment. Colony-forming units (CFU) were assessed to measure the bacterial load. Statistical Analysis Used: Data were analyzed using the one-way analysis of variance ( P < 0.05) followed by post hoc Tukey test to compare CFU reductions among groups. Results: All groups showed significant CFU reduction. The Mangosteen with Chitosan group demonstrated the highest reduction (84.57%), followed by Chitosan (79.99%), Mangosteen (77.06%), and Ca(OH) 2 . No adverse effects were reported. Conclusions: The combination of mangosteen and chitosan showed the most effective antimicrobial activity, suggesting its potential as a natural and effective intracanal medicament.
Authors: Lakshika Sharma, Dakshita Joy Sinha, Rohit Paul, Shipra Singh, Anisha Deb, Jayati Pandey
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4411965295