Year: 2026
Type: article
Abstract: Mutations of EIF2AK4 , which encodes the eIF2α kinase GCN2, cause a severe inherited form of pulmonary hypertension called pulmonary veno-occlusive disease (PVOD). Some pathogenic variants of GCN2 are amenable to pharmacological reactivation by low concentrations of ATP-pocket binding inhibitors. Kinase inhibition at modestly elevated concentrations limits the clinical utility of these drugs against PVOD. We therefore performed an in cellulo chemical screen for GCN2 activators and identified three structurally distinct compounds with low micromolar stimulatory activities. Unlike previously described GCN2 activators, one of these molecules activated GCN2 independently of GCN1. Modelling supported by structure activity screens suggested it binds within the ATP-pocket of GCN2, but unlike existing ligands does not protrude inward into the allosteric pocket or outward into the solvent. This overcomes a key requirement of other GCN2 activators.
Source: eLife
Authors: JiaYi Zhu, Giulia Emanuelli, Glenn R Masson, Vanesa Vinciauskaite, Henriette Willems, Peiqiao Cai, Andrew Lim, Christopher Alan Brown, David Winpenny, Murray Clarke, Rebecca Gilley, Fergus Preston, Jordan CJ Wilson, Aldo Bader, Taufiq Rahman, Joseph E Chambers, John Skidmore, Nicholas W Morrell, Stefan J Marciniak
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,500
Funders: Royal Papworth Hospital NHS Foundation Trust, University of Dundee, UK Research and Innovation, British Heart Foundation, British Lung Foundation, Asthma and Lung UK, Tenovus, University of Cambridge, Cambridge Trust, Evelyn Trust, Directorate for Biological Sciences, Medical Research Council, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, Cambridge Institute for Medical Research, University of Cambridge, NIHR Cambridge Biomedical Research Centre
Awards: G1213859024, G1346166066, G139696651, G1521853241, G1600286315, G1682416977, G1804826484, G2165920734, Research Excellence (round 3), G3584577881, Construction of a High-Throughput Hydrogen Deuterium Exchange Mass Spectrometry Platform, Targeting PPP1R15 in malignancy, G4381252014, G5028607417, G6077662935, G6641991021, G7121181839, G763011351, Effects of alpha1-antitrypsin polymerisation on organelle structure and fluidity in hepatocytes, G8343216130, G8792702861
OpenAlex ID: W4411975153