Work

In Vitro Culture of Vitrified Immature Mouse Testicular Tissue in The Presence of N-acetylcysteine Antioxidant.

Year: 2025

Type: article

Abstract: BACKGROUND: culture of cryopreserved testicular tissue. Optimizing the culture conditions is essential for reducing tissue damage caused by oxidative stress produced during cryopreservation and culture. Our objective was to improve the culture conditions of vitrified immature mouse testicular tissue by using N-acetylcysteine (NAC) antioxidant. MATERIALS AND METHODS: ) gene expression, and Caspase-3 protein expression were assessed. Moreover, the malondialdehyde (MDA) level was measured in the culture media. RESULTS: gene expression were significantly increased. CONCLUSION: culturing of human prepubertal testicular tissues.

Source: PubMed

DOI: 10.22074/ijfs.2025.2039641.1749

Authors: Parmiss Nikoosokhan, Zeinab Ghezelayagh, Samira Hajiaghalou, Alireza Alizadeh Moghadam Masouleh, Bita Ebrahimi

Institutions: University of Science and Culture, Royan Institute

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W4411977597