Year: 2025
Type: article
Abstract: Ligation is a common treatment for the resulting DNA nanostructures to gain extra stability. In this study, we aim to utilize ligation as an active process in self-assembly instead of post-assembly stabilization. Our investigation focuses on constructs with transiently paired segments. Transient base pairing fails to hold up the assembled complex, but ligation treatment turns the transient base pairing into permanent ones and thus induces the desired self-assembly. We apply the method to a number of assembly tasks, leading to the successful construction of discrete and extended DNA nanostructures. Furthermore, we apply the ligation-based method in the hierarchical assembly of preformed DNA nanostructure units into higher-order superstructures.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkaf570
Authors: Qishu Zhang, Zhengyang Sun, Yaduo Wang, Lei Xue, Jianqiu Zhao, Yue Shen, Bryan Wei, Wen Wang
Institutions: Tsinghua University, BGI Research
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,992
OpenAlex ID: W4412034311