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Structure‐Guided Engineering of Thermodynamically Enhanced SaCas9 for Improved Gene Suppression (Adv. Mater. 26/2025)

Year: 2025

Type: article

Abstract: Thermostable Protein Engineering In article number 2404680, Trung Thanh Thach, Jaekyung Hyun Yong Ho Kim, and co-workers introduce a structure-guided engineered SaCas9 variant with enhanced thermostability and compactness, enabling efficient gene suppression through nonviral delivery. The engineered sdCas9-KRAB-R system demonstrates superior nuclear localization and in vivo knockdown efficiency, showcasing its potential as a robust tool for CRISPR interference and targeted genomic regulation.

Source: Advanced Materials

DOI: 10.1002/adma.202570177

Authors: Eun Sung Kang, Nam Hyeong Kim, Hyun‐Kyoung Lim, Hyeyeon Jeon, Kayoung Han, Young Hyun No, Kyungtae Kim, Zinah Hilal Khaleel, Dongsun Shin, Kilho Eom, Jiyoung Nam, Bok‐Soo Lee, Han‐Joo Kim, Minah Suh, Jaecheol Lee, Trung Thanh Thach, Jaekyung Hyun, Yong Ho Kim

Institutions: Sungkyunkwan University, Institute for Basic Science

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W4412039923

FWCI:

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