Antimigratory, Anti‐Invasive and Anti‐Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β‐Catenin Pathway
Year: 2025
Type: article
Abstract: BACKGROUND: This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved. METHODS: In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue. RESULTS: Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. In vivo, Bergenin alleviated paw swelling, arthritis score, and synovial pathology in AIA rats, reducing pro-inflammatory factors. CONCLUSIONS: Bergenin exerts antimigratory, anti-invasive, and anti-inflammatory effects in rheumatoid arthritis by modulating the Wnt/β-catenin pathway, suggesting its potential as a therapeutic option for rheumatoid arthritis.
Authors: Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi
Institutions: Soochow University, Wuxi Ninth People's Hospital
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: Soochow University
OpenAlex ID: W4412095743