Work

Protein Isolation and Purification of Sea Cucumber (Holothuria sp. and Stichopus sp.) from Sepanjang Beach, Yogyakarta

Year: 2025

Type: article

Abstract: Holothuria sp. and Stichopus sp. at Sepanjang Beach, Yogyakarta were studied for protein isolation. The crude protein concentrations in the body wall and intestine of Holothuria sp. were 2.42% and 2.13%, while Stichopus sp. had 4.03% and 4.01%, respectively. Proteins identified through SDS-PAGE analysis included collagen, softenin, and actin. Purified protein concentrations in Holothuria sp. were 3.72 ± 0.04 μg mL⁻¹ (body wall) and 4.09 ± 0.05 μg mL⁻¹ (intestine), while Stichopus sp. had 3.98 ± 0.08 μg mL⁻¹ and 9.34 ± 0.48 μg mL⁻¹, respectively. This study supports nutraceutical development, identifying potential health-enhancing supplements and drugs.

Source: Journal of Tropical Biodiversity and Biotechnology

DOI: 10.22146/jtbb.11901

Authors: Budi Khoiri Ardiansyah, Tri Rini Nuringtyas

Institution: Universitas Gadjah Mada

Language: English

Topic: Echinoderm biology and ecology

Subfield: Aquatic Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Life below water

Open Access status: gold

Estimated APC paid: $150