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Figure 4 from Uncovering Novel lncRNAs Linked to Melanoma Growth and Migration with CRISPR Inhibition Screening

Year: 2025

Type: other

Abstract: XLOC_030781 CRISPRi knockdown and RNA-seq transcriptomic expression analysis in 501mel-dCas9-KRAB. A, Principal component analysis (PCA) of sgXLOC_030781 CRISPRi at days 3 and 5 after infection vs. day 0 negative control (n = 2). B, Volcano plot of significantly differentially expressed genes (red) upon sgXLOC_030781 knockdown at day 3; P value cutoff = 0.05 and log2 fc = 1. C, GO enrichment analysis of up- and downregulated gene sets upon sgXLOC_030781 knockdown at day 3. D, GSEA enrichment analysis of each top example of upregulated (ER to Golgi vesicle transport, left) and downregulated genes (DNA replication–dependent nucleosome assembly, right). E, Upstream TF analysis of up- and downregulated genes using ChEA upon sgXLOC_030781 knockdown at day 3.

DOI: 10.1158/2767-9764.29513961

Authors: Stavroula Petroulia, Kathryn Hockemeyer, Shashank Tiwari, Pietro Berico, Sama Shamloo, Seyedeh Elnaz Banijamali, Eleazar Vega-Saenz de Miera, Yixiao Gong, Palaniraja Thandapani, Eric Wang, Jeffrey L. Schloßhauer, Aristotelis Tsirigos, Iman Osman, Ioannis Aifantis, Jochen Imig

Language: English

Topic: Cancer-related molecular mechanisms research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4412112323

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