Figure 1 from Uncovering Novel lncRNAs Linked to Melanoma Growth and Migration with CRISPR Inhibition Screening
Year: 2025
Type: other
Abstract: LncRNA candidate selection for CRISPRi screen and expression of lncRNAs associated to melanomagenesis. A, Flow diagram of integrated RNA- and ChIP-seq for the preselection of lncRNAs. Selection parameters are FDR < 5%, log fc > 0.75, and CPM > 1 in higher expressed cell type, either histone mark (H3K4me3 or H3K27ac) in the higher expressed cell type; no mono-exonic lncRNAs and log fc ≥ 0.5 between investigated cell type and either WM1361a or 501mel. B, Heatmap of differentially expressed lncRNAs by RNA-seq in patient/donor-derived melanocytes (n = 4) and metastatic STCs (BM, brain metastasis, n = 5; LN, lymph node metastasis, n = 4) and melanoma cell lines 501mel (n = 4) and WM1361a (n = 3). C, Heatmap showing differential lncRNA expression in the SK-Mel239 parental melanoma cell line versus BRAF and 1059 MEKi-resistant cell line (n = 2). D, Boxplot showing the log2 CPM expression of selected melanoma-associated lncRNAs SAMMSON, MEG3, H19, and PCAT1 in melanocytes (n = 4), brain metastasis (n = 5), and LN STCs (n = 4) detected by RNA-seq. DE, differentially expressed; Mets, metastasis; TSS, transcription start site.
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4412112355