The Human Papillomavirus (HPV) Laboratory e-Manual: A comprehensive guide for HPV testing and research
Year: 2025
Type: article
Abstract: BACKGROUND: Human Papillomavirus (HPV) vaccination and HPV-based cervical cancer screening are central pillars of the World Health Organization (WHO) global cervical cancer elimination strategy. The WHO HPV Laboratory Manual, published in 2009, has provided essential guidance to promote an internationally comparable quality of HPV testing for many years. As the development in this area is rapid, the Global Network of National HPV Reference Laboratories considered that there is a need for an updated HPV Laboratory e-Manual to serve as a comprehensive and interactive resource for professionals engaged in quality-assured HPV testing for research and/or HPV-based cancer control. CONTENT: The HPV Laboratory e-Manual covers key areas, including laboratory quality assurance, HPV taxonomy and risk association, collection and handling of specimens, nucleic acid extraction, HPV detection and typing, HPV serology, data management, and the use of international standards. It provides up-to-date protocols and best practices to enhance accuracy and reliability of HPV testing. Interactive features allow for real-time updates, making it a dynamic resource for laboratories worldwide. The e-Manual is freely available at: https://www.hpvcenter.se/hpv-laboratory-manual/. COLLABORATORS: The e-Manual has been developed by international experts from 11 countries, including contributors from the International HPV Reference Center (IHRC, Sweden), the CDC's Global HPV Reference Laboratory (USA), and multiple National HPV Reference Laboratories (NRLs). The standard procedure for writing a chapter was that 2 NRLs authored the chapter and 1 other NRL reviewed it. CONCLUSION: The HPV Laboratory e-Manual represents a step toward global harmonization in laboratory methodologies for HPV testing, underpinning both research and cervical cancer control efforts.
Source: Journal of Clinical Virology
Authors: Laila Sara Arroyo Mühr, Carina Eklund, Camilla Lagheden, Rita Mariel Correa, María Dolores Fellner, Maria Alejandra Picconi, Nazlı Songur, Murat Gultekin, Kate Cuschieri, Jean-Luc Prétet, Quentin Lepiller, Alice Baraquin, Steffi Silling, Kristiane Søreng, Milan Stosic, Gro Kummeneje Presthus, Marc Arbyn, Michael Peeters, Steven Van Gucht, Elizaveta Padalko, Taeyang Chin, Kaat Kehoe, Ana Rita Pereira, Nina Redzic, Davy Vanden Broeck, Monica Molano, Gerald L. Murray, Suzanne M. Garland, Ligia A. Pinto, Dianna E. Wilkinson, Mario Poljak, Joakim Dillner
Institutions: Karolinska University Hospital, Karolinska Institutet, Administración Nacional de Laboratorios e Institutos de Salud, Hacettepe University, Edinburgh Royal Infirmary, MRC Centre for Reproductive Health, University of Edinburgh, Centre National de la Recherche Scientifique, Centre Hospitalier Universitaire de Besançon, Laboratoire Chrono-Environnement, Université Marie et Louis Pasteur, University of Cologne, University Hospital Cologne, Akershus University Hospital, Sciensano (Belgium), Ghent University Hospital, Royal Women's Hospital, The University of Melbourne, Murdoch Children's Research Institute, Frederick National Laboratory for Cancer Research, Medicines and Healthcare products Regulatory Agency, University of Ljubljana
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,210
Funders: U.S. Department of Health and Human Services, Bill and Melinda Gates Foundation, World Health Organization, Centre International de Recherche sur le Cancer, Cancerfonden, Javna Agencija za Raziskovalno Dejavnost RS, National Institutes of Health, International Arctic Research Center, University of Alaska, Fairbanks, National Health and Medical Research Council, Horizon 2020, National Cancer Institute
OpenAlex ID: W4412392479