Work

Tailored solution environment for protein crystallization: tuning solubility, nucleation, and growth by urea and salt

Year: 2025

Type: article

Abstract: , enhances both nucleation and growth compared to salt alone. Overall, our results reveal how urea and salt independently govern thermodynamic and kinetic factors. We propose that tuning the solution environment through salt and nonspecific additives such as urea provides a general strategy to optimize crystallization conditions for globular proteins.

Source: Physical Chemistry Chemical Physics

DOI: 10.1039/d5cp01614b

Authors: T. Hamacher, F. Platten

Institutions: Heinrich Heine University Düsseldorf, Forschungszentrum Jülich

Language: English

Topic: Crystallization and Solubility Studies

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Funder: Deutsche Forschungsgemeinschaft

Award: Das Phasenverhalten von Proteinlösungen in elektrischen Feldern