Work

Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation.

Year: 2025

Type: article

Abstract: Objective: L.) root extract and carbenoxolone on PC12 cells cultured in the serum/glucose deprivation (SGD) condition. Materials and Methods: methanol extract (5-320 µg/ml) and carbenoxolone (0.5-32 µM) for 2 hr before being deprived of serum/glucose. Protection against cytotoxicity, increase in reactive oxygen species (ROS), and apoptosis was analyzed with resazurin, dichlorofluorescein diacetate (DCFH-DA), and western blot, respectively. Results: methanol extract and carbenoxolone at 5-20 µg/ml significantly prevented from the Poly (ADP-ribose) polymerase (PARP) and caspase-3 cleavage. Conclusion: model of ischemia. Overall, it seems that pretreatment with the licorice extract and carbenoxolone may potentially slow the progression of brain ischemia.

Source: PubMed

DOI: 10.22038/ajp.2024.25252

Authors: Hossein Hosseinzadeh, Elham Ramazani, Soheyla Bakhshi, Zahra Tayarani-Najaran

Institutions: Mashhad University of Medical Sciences, Yazd University

Language: English

Topic: Pharmacological Effects of Natural Compounds

Subfield: Pharmacology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Mashhad University of Medical Sciences