In-situ anchoring an Ag-based metal-organic framework onto carboxymethylcellulose hydrogel film: A potential bio-platform for antibiotic-free wound dressing
Year: 2025
Type: article
Abstract: An efficient method for preventing bacterial infections of wounds is to prepare an antibacterial agent with the right mechanical, antibiotic, and water vapor permeability features. In this work, a novel method was applied to develop a nanocomposite bio-platform by in-situ anchoring of silver-based metal-organic frameworks (Ag-MOFs) onto the carboxymethylcellulose (CMC) hydrogel film. In this regard, CMC films were prepared using citric acid and glycerol as a crosslinker and plasticizer, respectively. Subsequently, Ag-MOFs were synthesized directly on the film surface via immersion-coordination of Ag⁺ ions with 2-aminoterephthalic acid, eliminating the need for additional stabilizers. Various techniques (i.e., FT-IR, XRD, SEM, EDX-mapping, AFM, etc) were utilized that verify the successful synthesis of CMC/Ag-MOF nanocomposite. The results of in-vitro cytotoxicity and antibacterial assays demonstrated that the CMC/Ag-MOF nanocomposite exhibited acceptable cytocompatibility, maintaining cell viability above 60% at a concentration of 8 mg/mL against human skin fibroblast cells (HFF-2). Moreover, it showed significantly enhanced antibacterial performance, with inhibition zones against S. aureus and E. coli increasing by approximately 66.7% and 87.5%, respectively, compared to the pure CMC film. The obtained results recommended CMC/Ag-MOF hydrogel films as a potential antimicrobial dressing.
Institution: University of Tabriz
Language: English
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,790
Funder: University of Tabriz
OpenAlex ID: W4412458883