Work

Protocol for performing and analyzing a live-cell imaging-based microglia phagocytosis assay using AI on the Olympus ScanR system

Year: 2025

Type: article

Abstract: Various neurological disorders show dysregulation of microglial function, including phagocytosis, necessitating accurate and efficient analysis. Here, we present a protocol using our custom-trained neural network (AIstain) in the Olympus ScanR environment for easy-to-use workflows of microglial phagocytosis assays. We describe steps for preparing microglia and setting up the microscope. We then explain the workflow of live-cell imaging and usage of AI in the data analysis. This protocol facilitates the preparation of phagocytosis assays and improves accuracy in data analysis. For complete details on the use and execution of this protocol, please refer to Zähringer et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103846

Authors: Alexander Zähringer, Janaki Manoja Vinnakota, Tobias Wertheimer, Marie Follo, Robert Zeiser

Institutions: University of Freiburg, University Medical Center Freiburg

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550