Work

Membrane adsorber design for lentiviral vector recovery

Year: 2025

Type: article

Abstract: 80% at t = 100 min). Narrower elution ranges were shown, with LV eluted <450 mM NaCl. The best-performing prototype, DR2, exhibited a ∼3-fold higher functional product recovery than standard Q-membranes for LV encoding GFP (50%) and CAR (73%) transgenes. At large-scale, downstream processes using DR2 membranes showed a 3.5-fold improvement in functional product recovery at drug substance (43%) compared to a standard Q-membrane process (12%). These results demonstrate that adsorbents designed for lentiviral vectors significantly enhance downstream recoveries.

Source: Molecular Therapy — Methods & Clinical Development

DOI: 10.1016/j.omtm.2025.101533