Synergistic effect of grape seed extract in combination with green coffee bean for the treatment of diabetes mellitus
Year: 2025
Type: article
Abstract: The proposed project successful attempt has been made to synergistic effect of grape seed extract in combination with green coffee bean for the treatment of diabetes mellitus. I started process plant extraction is a modern technique that offers a rapid method for extraction phytoconstituents. Coffee arabica extract percentage yield 11.44% and grape seed extract percentage yield 10.23% mixture of these two extract were taken 1:1 ratio for the present study to find the synergistic anti diabetic activity phytochemical study conducted to investigate the present various components CBGSC and process of Alpha-Amylase inhibition activity extract lower that the standard acarbose[IC50=9.08µg/ml]grape seed extract was used alone AAI rate 24.20ug/ml and green coffee extract AAI-rate 20.13µg/ml combination of extract observed at 92.56%at360ug/ml IC50 was found to be 17.33µg/ml. alpha-glucosidase inhibition GS extract concentration 88.72% AGE.IC50 value 17.04µg/ml. GC Extract concentration 89.61%AGE.IC50 value 15.42µg/ml. Molecular docking analysis was conducted human alpha glucosidase binding energy and beta -D-glucopyranosyl binding energy 9.39Kcal/mol . and inhibition constant 130.88nm. Binding site of ligand hydroxyl group of Ligand -binding distance LLN167o-H-o -3.2, ASP199O-H-o-3.1, ASG411N-H-o-2.6 All the process are followed by as per WHO and ICH guidelines
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4412466722