Work

HSQC/F 1 ‐PSYCHE TOCSY NOAH Supersequence for High‐Resolution NMR Analysis of Urine Metabolites

Year: 2025

Type: article

Abstract: ABSTRACT Accurate assignment of metabolites is the backbone of metabolomics studies. Two‐dimensional (2D) NMR plays a critical role in the accurate assignment of metabolites. Characterization of 2D spectra such as 1 H– 13 C HSQC and 1 H– 1 H TOCSY combined with database queries enables reliable metabolite identification for metabolic profiling and biological interpretation. However, recording a high‐quality 1 H– 13 C HSQC spectrum at 13 C natural abundance in biofluids requires extensive NMR signal averaging, often taking up to 24 h. Reducing the number of t 1 increments or scans is not useful in metabolomics as it compromises the sensitivity needed to detect low‐abundance metabolites. “NMR by Ordered Acquisition using 1 H detection,” or NOAH, supersequences are ideally suited for accelerated data collection in biofluids. Instead of shortening individual experiments, NOAH enables the simultaneous acquisition of multiple 2D experiments without compromising sensitivity. The principle of NOAH lies in utilizing the undisturbed magnetization from one experiment (e.g., 1 H– 13 C HSQC) for subsequent experiments (e.g., 1 H– 1 H TOCSY) within the same scan. Previous studies have demonstrated the utility of the HSQC + TOCSY NOAH‐2 supersequence for metabolomics applications. Nevertheless, due to the complexity of biofluids, even regular 2D TOCSY spectra often suffer from signal overlap, arising from numerous metabolite peaks, multiplet structures, and limited 1 H chemical shift dispersion. The pure shift F 1 ‐PSYCHE TOCSY experiment addresses this challenge by offering a single peak per resonance, thereby greatly reducing signal overlap. In this work, we present HSQC + F 1 ‐PSYCHE TOCSY NOAH‐2 supersequence for the analysis of human urine.

Source: Magnetic Resonance in Chemistry

DOI: 10.1002/mrc.70013

Authors: Aditi Pandey, Nidhi Tiwari, Amrita Sahu, Bikash Baishya

Institutions: Centre of Biomedical Research, Academy of Scientific and Innovative Research

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Department of Science and Technology, Ministry of Science and Technology, India, Indian Council of Medical Research, University Grants Commission, Academy of Scientific and Innovative Research

Awards: G4958615209, G6830978983