Work

A unique three-enzyme cascade mediates efficient regioselective and stereospecific epoxytetrahydrofuran ring formation in deoxyverrucosidin biosynthesis

Year: 2025

Type: article

Abstract: a flavin-containing monooxygenase-mediated epoxidation on the polyene chain, followed by rearrangement with an epoxide expandase and a second epoxidation on the resulting 2,5-dihydrofuran ring with a cytochrome P450 enzyme. This catalytic cascade differs clearly from the formation of dioxybicyclooctane or tetrahydrofuran motifs in other α-pyrone-containing metabolites, involving one flavin-containing monooxygenase and one hydrolase for up to two rounds of epoxide ring formation and expansion.

Source: Chemical Science

DOI: 10.1039/d5sc03423j

Authors: Hui-Ling Wei, Xiao-Ling Chen, Yu Dai, Li Yang, Shu-Ming Li

Institutions: Philipps University of Marburg, Chinese Academy of Tropical Agricultural Sciences

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: diamond

Funders: Philipps-Universität Marburg, Deutsche Forschungsgemeinschaft, China Scholarship Council, Chinese Government Scholarship

Awards: G1524764220, G1713080561, G252668833, G4153166347, G5629188867