Work

Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody.

Year: 2025

Type: article

Abstract: well, respectively. The OD values < 0.70 were considered positive, and values ≥ 0.70 were negative for in-house kit base on standard controls. Statistical analysis base on 80 serum samples showed the 96.66% sensitivity, 100% specificity, 100% positive predictive value, 90.90% negative predictive value, 97.50% accuracy, and 98.33% reliability for serum samples for two commercial and in-house kits. The SPCE developed based on IgY antibody is a suitable alternative for the detection of antibodies after vaccination against type A FMDV with high sensitivity and specificity. The present research demonstrated the possibility of commercial development of the SPCE kit using IgY antibodies for the detection of FMDV antibodies in serum samples with adequate sensitivity and accuracy.

Source: PubMed

DOI: 10.30466/vrf.2024.2025842.4226

Authors: Zahra Ivani, Behzad Hemati, Naser Harzandi, Seyed Mahmoud Azimi, Mohammad Mehdi Ranjbar

Institutions: Islamic Azad University, Karaj, Razi Vaccine and Serum Research Institute, Agricultural Research & Education Organization

Language: English

Topic: Animal Disease Management and Epidemiology

Subfield: Agronomy and Crop Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Razi Vaccine and Serum Research Institute