Work

A mechano-enzymatic method to produce nano/microcellulose with ancestral endoglucanase. A comparative study

Year: 2025

Type: article

Abstract: Given the abundance of cellulose and the potential of nano/microcellulose, here in this work we analyze the prospects of five cellulosic sources for obtaining nano/microcellulose. We assess the impact of three different enzymes (ancestral reconstructed endoglucanase, cellulase and α-amylase) on a mechano-enzymatic process. The characterization of the resulting material has been carried out using spectroscopic, diffraction, thermal and morphological techniques. The results indicate that enzymatic extraction slightly contributes to the increase in nano/microcellulose extraction efficiency, reaching ∼12.5% with endoglucanase, which is higher than the ∼8% achieved without the enzyme, increases the degree of crystallinity of the sample without altering the structure and chemical composition and hardly decreases the crystallite size. These findings contribute to understanding the role of enzyme technology in the development of novel sustainable cellulose based materials, when pursuing economic and environmental benefits.

Source: Nanoscale Advances

DOI: 10.1039/d5na00524h

Authors: Ane Rivas-Zúñiga, Arantxa Eceiza, Borja Fernández-d’Arlas

Institutions: University of the Basque Country, CIC nanoGUNE, Siemens Gamesa Renewable Energy (Spain)

Language: English

Topic: Advanced Cellulose Research Studies

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $1,962