Cinnamaldehyde ameliorates obesity-induced nephropathy in C57BL/6 mice via modulation of AMPK/ACC and NF-kB pathways.
Year: 2025
Type: article
Abstract: Objectives: Chronic kidney disease (CKD) is a life-threatening condition often resulting from obesity and other pathologies. The present study assesses the nephroprotective effect of Cinnamaldehyde against high-fat diet (HFD) obesity-associated nephropathy in rodents. Materials and Methods: The molecular docking analysis on AMPK & NF-kB was carried out to identify possible targets of Cinnamaldehyde. In preclinical study, 4-week-old C57BL/6 mice (18-20 gm) were fed a conventional diet or HFD for 12 weeks After the fifth week of HFD intervention, mice were divided into six groups (n=10): vehicle group; HFD group; HFD+CA (20 mg/kg); HFD+CA (40 mg/kg); HFD+Orlistat (10 mg/kg); and CA Perse (40 mg/kg) treated orally for 49 days. On day 84, mice were fasted overnight, and urine and blood were collected for various biochemical analyses. Animals were sacrificed, and kidneys were removed for histopathology and immunohistochemistry. Results: <0.001) reduction in KIM-1, type-IV collagen, IL-18, and NGAL urinary levels. Further, it produced a significant (P<0.001) increase in urine creatinine, serum adiponectin, and kidney SOD, GSH, GST, and GPx. Immunohistology indicated suppressed NF-kB and activated AMPK/ACC pathways. Histopathology showed improvement in glomerular inflammation, tubular injury, and degeneration in kidney tissue. Conclusion: Cinnamaldehyde significantly protects against obesity-associated nephropathy in C57BL/6 mice by HFD via modulating the AMPK/ACC and NF-kB pathways.
Source: PubMed
Authors: Himani Gupta, Uma Bhandari
Institution: Jamia Hamdard
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4412650411