Work

Spermine: A prospective treatment for high glucose-induced myocardial fibrosis in db/db mice.

Year: 2025

Type: article

Abstract: Objectives: This study explored the molecular mechanism by which exogenous spermine attenuates diabetic cardiomyopathy (DCM)-induced myocardial fibrosis. Materials and Methods: experiments. The levels of total cholesterol (TC), triglycerides (TG), creatine kinase isoenzyme (CK-MB), troponin I (cTnI), and lactate dehydrogenase (LDH) were measured. Heart function and collagen deposition were assessed using echocardiographic analysis, Masson staining, and Sirius red staining. Cell proliferation and migration were analyzed using EdU and transwell assays. Relevant protein expression was evaluated by immunohistochemistry and western blot. Results: , high glucose conditions led to increased proliferation of cardiac fibroblasts. SSAT, α-SMA, TGF-β1, Collagen-I/III, MMP-2, MMP-9, p-Smad-2, TβRI, and TβRII were up-regulated, while ornithine decarboxylase (ODC) expression was down-regulated. Interestingly, these changes were reversed in the HG + SP group. Conclusion: Our findings demonstrate that SP reduces collagen synthesis and secretion by inhibiting the TGF-β1/Smads signaling pathway. These results provide new insights into potential therapeutic approaches for DCM.

Source: PubMed

DOI: 10.22038/ijbms.2025.83464.18060