Biochemical characteristics of pro- and antioxidant potential of plant extracts in dairy environment
Year: 2025
Type: article
Abstract: Actuality.Due to the high content of antioxidants, vitamins, minerals in plant extracts, their use for inhibiting oxidative processes is gaining popularity.The antioxidant efficacy of aqueous extracts from basil seeds, grape leaves, robinia flowers and wheat grains can be investigated using cow and goat milk as a research medium.The purpose of the study.To determine the content of secondary metabolites in plant raw materials and to evaluate the pro-and antioxidant potential of aqueous plant extracts in dairy environment. Material and methods.Native cow and goat milk.Water extracts from grape leaves (Vitis vinifera L.), robinia flowers (Robinia pseudoacacia L.), basil seeds (Ocimum basilicum L.) and wheat grains (Triticum sp.).The aqueous extracts were prepared in a 3 : 10 ratio, followed by extraction at 90 ℃ temperature for 40 minutes.Analysis of secondary metabolites was carried out by reverse phase high performance liquid chromatography.To prepare extracts for HPLC analysis 0,3 g of finely ground materials were mixed with 20 mL of methanol (Merck, Germany) and the obtained mixtures were treated with ultrasound at 50 ℃ for 120 min.Syringe filters with PTFE membranes (0,22 μm pore size) were used for filtration of obtained extracts.The content of oxidation products of lipids and proteins, vitamin C, SH groups, reduced glutathione, catalase activity and ascorbate peroxidase were determined spectrophotometrically.Plant extracts were added in a volume of 3% of the milk volume.№ 2, 2025 133 Біологія.Фармація Research results.Basil seeds have been found to have the highest levels of triglycerides and monoterpenoids, giving it a potential anti-inflammatory, bactericidal, anthelmintic and antispasmodic effect.Robinia flowers have the highest glycoside levels of anthocyanin, 4',7-dihydroxyflavone, apigenin, luteolin, and hydroxycinnamic acid derivatives, demonstrating their potent antioxidant potential and high levels of adaptation to biotic and abiotic stress.Instead, the highest levels of kaempferol and quercetin glycosides, simple phenols and chlorophyll A and B catabolites were found in the grape leaves.Such results demonstrate its likely anti-inflammatory and immunomodulatory effect.The poorest in the content of secondary metabolites was wheat grain. Фітотерапія. ЧасописAlso, as a result of the study, the most effective in terms of reducing the intensity of oxidation processes in milk and increasing its antioxidant potential was the extract from basil seeds.Extract from wheat grains showed the worst result in terms of increasing the antioxidant potential of milk and reducing the intensity of oxidation of proteins and lipids. Conclusion.The results obtained with milk may indicate a significant antioxidant potential of the studied plant extracts.These results can be used in crop production, as well as in the medical, pharmaceutical or food industries.
Source: Fìtoterapìâ
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4412677342