Work

The Therapeutic Treatment of Pepitem Increases the Protein Expression of Sirt1 in a Mouse Model of Experimental Autoimmune Encephalomyelitis (eae) as a Model for Human Ms

Year: 2024

Type: article

Abstract: Objective: To investigate the effect of the prophylactic and therapeutic treatment of the immunopeptide PEPITEM on the protein expression of SIRT1 in a mouse model of experimental autoimmune encephalomyelitis (EAE) as a model for human MS. Methods: Using C57BL/6 female mice, we dosed the PEPITEM in the EAE model via intraperitoneal injections either prophylactically or therapeutically. The disease was induced using MOG35-55 and complete Freund's adjuvants augmented with pertussis toxin. The EAE score was recorded daily until the end of the experiment (21 days). A Western blot analysis was performed on the brain lysate to measure the protein concentration of SIRT1. Results: The therapeutic treatment with PEPITEM increased the protein expressions of SIRT1 on the EAE mice whereas the prophylactic injections did not affect the protein expression of SIRT1. Conclusion: Collectively, the therapeutic treatment with PEPITEM suggesting anti-inflammatory effect of PEPITEM on the brain damage in EAE mice which offers a novel and safe strategy for drug therapy in MS, opening new avenues for research and treatment.

Source: LIFE International Journal of Health and Life-Sciences

DOI: 10.20319/lijhls.2024.10.2636

Author: Mohammed Alassiri

Institutions: King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center

Language: English

Topic: Multiple Sclerosis Research Studies

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: King Saud University, King Abdullah International Medical Research Center