Inhibition of Nuclear factor kappa B ameliorates bone destruction and osteoclastogenesis in collagen-induced arthritis mice
Year: 2025
Type: article
Abstract: Background: We aimed to investigate the effect of interleukin-1β (IL-1β)/nuclear factor ϰB (NF-ϰB) pathway on osteoclastogenesis and inflammatory bone destruction in a mouse model of collagen-induced arthritis (CIA). Methodology: DBA/1 mice were divided into CIA group, NF-ϱB inhibitor group and control group. The degree of paw edema was measured and the score of paw arthritis was assessed. mRNA levels of IL-1β, matrix metalloproteinase-1 (MMP-1), tumour necrosis factor-α (TNF-α) levels, anti-tartrate acid phosphatase (TRAP), matrix metalloproteinase-9 (MMP-9), tissue proteinase K (CtsK) and integrin β3 (β3-Integrin) in the joints of mice were determined. Results: The paw edema score at 18-30 d and the paw arthritis score at 12-30 d in CIA group were higher than those in control group, and the paw edema score and paw arthritis score at 24-30 d in NF-kB inhibitor group were lower than those in CIA group. mRNA levels of IL-1β, MMP-1, TNF-α in serum and TRAP, MMP-9, CtsK and β3-Integrin in joint tissues in CIA mice were higher than in controls. NF-ϰB inhibitor treatment significantly decreased the above mRNA levels both in serum and joint tissues. Conclusion: NF-ϰB pathway inhibition can ameliorate bone destruction in the foot joints of CIA mice, which may be related to the reduction of inflammatory bone destruction and osteoclastogenesis.
Source: African Health Sciences
DOI: 10.4314/ahs.v25i1.17
Authors: Jiangtao Guo, Xiaoli Ma, Lili Wu, Wei Zhao, Yan Zhang, Chunfang Hao, Yashan Yang, Zhe Yin, Tianyu Xu, Yingqiang Zhang, Yinyan Guo, Xuqing Cao
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4412755836