Application value of metagenomic next-generation sequencing of bronchoalveolar lavage fluid in pathogen detection and diagnostic efficiency of acute exacerbation of bronchiectasis
Year: 2025
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: To investigate pathogen detection performance and diagnostic efficacy of bronchoalveolar lavage fluid (BALF) metagenomic next-generation sequencing (mNGS) in patients with acute exacerbation of bronchiectasis (AE-bronchiectasis). METHODS: A retrospective analysis was conducted on 78 patients with AE-bronchiectasis admitted to the First Affiliated Hospital of Guangxi Medical University from March 2020 to December 2023. Pathogen detection rates and diagnostic efficacy of conventional culture detection and BALF mNGS group were compared. Seventy-six patients diagnosed as positive by the gold standard were further stratified by bronchiectasis severity index (BSI) into mild-to-moderate and severe groups to analyze differences in pathogen profiles. RESULTS: in the severe group (51.06%) was significantly higher than that in the mild-to-moderate group (27.59%), while the detection rate of human herpesvirus 7 was significantly higher in the mild-to-moderate group (24.14%) compared to the severe group (4.26%) (all P<0.05). CONCLUSION: BALF mNGS demonstrates clear advantages over conventional methods in pathogen detection for AE-bronchiectasis, offering significantly better detection rates and diagnostic efficiency.
DOI: 10.62347/wolq1347
Author: Zaiqing He
Institution: Guangxi Medical University
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4412775638