Supplementary Figure 6 from CTG-Initiated Cryptic Peptide Translation Up- and Downstream of a Canonical ATG Start Codon Is Enhanced by TLR Stimulation and Induces Tumor Regression in Mice
Year: 2025
Type: supplementary-materials
Abstract: Supplementary Figure 6: Translational readthrough events contributed to enhanced tumor rejection in vivo.(A+B) A second batch of AE17-NC, AE17-CTGup, AE17-CTGdown and AE17 cells expressing additional CTGdown-out construct (AE17-CTGdown-out) were generated.2x10 6 corresponding AE17 cells were injected subcutaneously into the hind flank of C57BL/6J mice, and tumor progression was followed by regular caliper measurements.Average tumor sizes plotted against time after tumor injection (A) and a Kaplan-Meier survival curve (right panels) are shown (B) (n=8).(C) Same batch of AE17-NC, AE17-CTGup, AE17-CTGdown and AE17-CTGdown-out injected in vivo were cocultured with 1x10 5 OT-1s for 4 hours in vitro to evaluate cryptic H-2Kb/S8L presentation.OT-1 T-cell activation was determined by intracellular cytokine staining for IFN-γ and subsequent flow cytometry.Percentage of CD8+ IFN-γ+ OT-I T-cells are displayed as a measure of OT-I T-cell activation.Indicated
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4412818513