Year: 2025
Type: article
Abstract: –Chitinase enzyme has diverse applications in the field of biotechnology, due to high attention especially in the field of agriculture for bio control of phytopathogenic fungi and harmful insects. In the present study, bacteria were isolated from marine rhizosphere soil. Bacterial isolates were screened for chitinolytic activity and isolates were selected for Chitinase production in broth media. Most powerful isolates were recognized as Aeromonas hydrophila and were selected for additional study, based on enzyme production. Studies were conducted on the effects of media composition and various fermentation conditions for optimization of chitinase production. At 37 o C and pH 8.0 after 24-48 h of incubation by Aeromonas hydrophila, maximum production of chitinase is obtained. Among the substrates, colloidal chitin was the best for the strain. Regarding carbon sources, maltose was the best for isolated strain; while meat was found as the best nitrogen source for Aeromonas hydrophila. Among surfactants, SDS was the best which shows major enzymatic activity
Authors: NAIR SREECHA CHANDRAN, KOMAL PRIYA, SREEVENI ., DIYA G., PARIJAT BANDEKAR, SUHANA MERAJ, AISHWARYA J., RAKSHITHA PATIL V,, MOHAMMED ZABI
Institution: Visvesvaraya Technological University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4412823576