Work

Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research

Year: 2025

Type: review

Abstract: . Originally developed to study bacterial transcriptional regulators, these systems have evolved to support a wide range of applications, including interaction mapping, domain analysis, and high-throughput screening. This review surveys the development and expansion of B2H systems across diverse biological contexts. We highlight technical considerations, platform-specific innovations, and recent adaptations that extend the utility of B2H systems in both prokaryotic and eukaryotic protein interaction research environments. By comparing different B2H variants and discussing their integration with complementary approaches, we provide a framework for leveraging these systems in modern PPI discovery.

Source: Journal of Bacteriology

DOI: 10.1128/jb.00129-25

Authors: Rebecca M. Richardson, Steven M. Pascal

Institution: Old Dominion University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: National Institute of Allergy and Infectious Diseases

Awards: G2684570531, G5457586278