Work

A predisposing effect of HLA class II genes in celiac disease by skewing the naive CD4 + T cell receptor repertoire

Year: 2026

Type: article

Abstract: Polymorphisms of human leukocyte antigen (HLA) genes confer risks for many human diseases. For predisposing effects relating to T-cell receptor (TCR) recognition of peptide-HLA, the effect can be both selection of the TCR repertoire and preferential presentation of disease-driving epitopes. In celiac disease (CeD) HLA-DQ2.5 predisposes by presenting deamidated gluten peptides to CD4 + T cells that typically employ stereotyped TCRs. Here we analyzed whether genetic variants within the HLA and TR loci shape the naïve TCR repertoire. We sequenced the αβ TCR repertoires of naïve CD4 + T cells of 103 CeD subjects and 103 controls and performed gene usage quantitative trait loci analyses. The naïve CD4 + TCR repertoire was significantly affected by HLA and TRA and TRB polymorphisms. Individuals carrying the HLA-DQ2.5 allotype exhibited increased frequencies of TCR genes involved in stereotyped recognition of gluten epitopes thus demonstrating a disease-predisposing effect of HLA by selection of a disease-relevant TCR repertoire.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.2520636123