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CRISPR-Cas9 in Functional Genomics: Implications for Target Validation in Precision Oncology

Year: 2025

Type: article

Abstract: CRISPR-Cas9 has revolutionized functional genomics by enabling precise genome editing with unprecedented efficiency. This review focuses on the application of CRISPR-based platforms for target discovery and validation in precision oncology. Key topics include the design of genome-wide CRISPR screens, synthetic lethality identification, and CRISPR interference (CRISPRi) and activation (CRISPRa) methodologies. We highlight current case studies where CRISPR has led to the identification of novel therapeutic targets, discuss technical limitations such as off-target effects, and explore ethical considerations in clinical translation.

Source: Trends in Pharmaceutical Biotechnology

DOI: 10.57238/tpb.2025.153196.1026

Authors: Iman Fadhel Abdul-Hussin, Mays Hassan Obais Alkhalidi, Sharafaldin Al-Musawi, Lubna Abd Muttalib Alshalah, Mohsen Sheykhhasan

Institutions: Al-Qasim Green University, University of Babylon, Qom University of Medical Science and Health Services

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W4412878368

FWCI: 1.022

Cites:

Cited by: 4

Related to: 10