Work

CRISPR/Cas9-Driven Engineering of AcMNPV Using Dual gRNA for Optimized Recombinant Protein Production.

Year: 2025

Type: article

Abstract: larvae. These findings validate an effective strategy for developing minimized baculovirus genomes and demonstrate that dual-guide CRISPR/Cas9 editing is a rapid and precise tool for baculovirus genome engineering.

Source: PubMed

DOI: 10.3390/v17081041

Authors: Rocco Valente, Joaquín Poodts, Joaquín Manuel Birenbaum, María Sol Rodriguez, Ignacio Smith, Jorge Alejandro Simonin, Franco Uriel Cuccovia Warlet, Aldana Trabucchi, Salvador Herrero, María Victoria Miranda, Mariano Nicolás Belaich, Alexandra Marisa Targovnik

Institutions: Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, National University of Quilmes, Universitat de València

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación, Agencia Nacional de Promoción Científica y Tecnológica

Awards: G142616781, G2094735105, G5430992182, G6467286343, G6599142069, G6741982938

OpenAlex ID: W4412915813

FWCI:

Cites: 55

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Related to: 10