Targeted Blockage of CXCR4 on the Epithelial-Mesenchymal Transition Effect between Epithelial Ovarian Cancer
Year: 2025
Type: article
Abstract: To investigate the effect of targeted blockage of CXCR4 on EMT and tumour invasion and metastasis in epithelial Ovarian cancer (OC). The expression of CXCR4 was detected using SP immunohistochemistry in 55 cases of OC and 35 cases of benign tissue. CXCR4 and EMT markers, correlation analysis of the related factors, CXCR4 protein expression and the clinicopathological characteristics of patients with OC; The CXCR4 antagonist AMD3100, OVCAR3 detection by western blot CXCR4 and the expression of EMT-related protein; MTT method, real ability experiment and Transwell experiment were used to detect the invasion of OC cell proliferation and cell clone formation, cell clone formation ability and invasion ability. The expression of CXCR4 and EMT-related proteins in OC was significantly higher than in the control group. The expression of CXCR4 in patients with tumour grade, FIGO staging, lymph node metastasis, and ascites had a close relationship (P < 0.05). After AMD3100 treatment, cell proliferation, colony formation, and invasion abilities were significantly inhibited in OVCAR3 cells. AMD3100 inhibited proliferation, invasion, and metastasis of OC by blocking CXCR4 and regulating the expression of EMT-related proteins. CXCR4 promoted the proliferation of OC, and the invasion and metastasis process may be related to the pathogenesis of EMT. Therefore, CXCR4 may be a new target for the treatment of OC.
Source: Health
Authors: Jinjian Wu, Fang Wen, Yongxiang Chen, Qizhu Zhang, Dan Zi
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4412917017