The establishment of a micropropagation system for Dendrobium kingianum via somatic embryogenesis
Year: 2025
Type: article
Abstract: The Dendrobium kingianum is a highly valued ornamental plant belonging to the Orchidaceae family whose propagation relies on time-consuming stem cutting and protocorm-like body proliferation. To optimize its propagation system, roots, leaves, and lateral buds of Dendrobium kingianum plants were taken to investigate embryogenic callus induction and somatic embryogenesis. Embryogenic callus was induced on MS medium containing 0.2 mg/L NAA, 1.5 mg/L 6-BA, and 10−7 mol/L PSK when lateral buds served as explants. Many somatic embryos were generated on G4 medium (1.0 g/L Hyponex No.1 + 1.0 g/L Hyponex No.2 + 15 g/L sucrose + 3 g/L Phytagel + 2 mg/L 6-BA + 0.5 mg/L IBA + 10 g/L corn starch + 200 mL/L coconut water), which developed into robust seedlings after rooting. The original embryogenic calluses proliferated continuously for more than half a year, and the newly proliferated ones possessed the capacity to develop into seedlings, indicating an efficient propagation system for Dendrobium kingianum via somatic embryogenesis with potential for scale production.
Source: Agrobiodiversity
Authors: Mengling Liu, LingLing Yuan, Lifang Wu, Zhenhong Lu, Chunmei Yang, Chunlian Jin
Institutions: Yunnan University, Yunnan Academy of Agricultural Sciences
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4412917483