Work

Extraction of PCB18 (2,2',5-Trichlorobiphenyl) and Its Hydroxylated Metabolites from Animal Serum v1

Year: 2025

Type: preprint

Abstract: This protocol describes the extraction of 2,2′,5-trichlorobiphenyl (PCB18) and its monohydroxylated metabolites from mouse blood or serum using liquid-liquid extraction followed by solid-phase extraction cleanup. The levels of PCB18 and OH-PCB11s in the blood/serum samples are subsequently measured by gas chromatography-tandem mass spectrometry.

DOI: 10.17504/protocols.io.bp2l6y5xkvqe/v1

Authors: Xueshu Li, Sean Brobston, Hansjoachim Lehmler

Institution: University of Iowa

Language: English

Topic: Toxic Organic Pollutants Impact

Subfield: Health, Toxicology and Mutagenesis

Field: Environmental Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Environmental Health Sciences

Awards: PCB-mediated dysbiosis of the gut microbiome: A missing link in PCB-mediated neurodevelopmental disorders?, Iowa Superfund Research Program: Airborne PCBs: Sources, Exposures, Toxicities, Remediation, Environmental factors in pathobiology of dementia: the role of PCB exposure, microbiome, and tissue barrier dysfunction, Environmental Health Sciences Research Center, Molecular and Cellular Basis of PCB Developmental Neurotoxicity