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MACL-1 Breast Cancer Secretome Reprograms Fibroblasts to Enhance Inflammation and Tumor Progression

Year: 2025

Type: article

Abstract: Tumor-secreted factors reprogram the stromal microenvironment to facilitate cancer progression. This study investigates the effects of the MACL-1 breast cancer cell secretome on CCD19-Lu fibroblasts. Fibroblasts treated with the MACL-1 secretome exhibited increased proliferation, altered morphology, and upregulated expression of vimentin, NFκB, and fibroblast-activating protein, indicative of an inflammatory, activated phenotype. Additionally, elevated mitochondrial superoxide levels and an enrichment of proteases were detected in the secretome, but not in corresponding cell lysates. These findings suggest that the MACL-1 secretome promotes fibroblast activation and inflammation, underscoring its potential role in tumor-stroma interactions and breast cancer progression.

Source: Anais da Academia Brasileira de Ciências

DOI: 10.1590/0001-3765202520250146