Method Validation for Determination of Caffeine in Sidomulyo Robusta Coffee Beans Using Gas Chromatography-Flame Ionization Detector
Year: 2025
Type: conference-paper
Abstract: Caffeine, an alkaloid compound found in coffee, is widely known for its ability to stimulate the human central nervous system. The caffeine content in Sidomulyo robusta coffee was analyzed using a gas chromatography-flame ionization detector (GC-FID) with ultrasound-assisted extraction as a preparation method using methanol solvent for 21 minutes at 32°C. A TR-1MS column (15m x 0.25 mm ID x 0.25 μm film, non-polar column 100% dimethyl poly-siloxane) was used for the GC analysis. The injector temperature was set at 260°C and the detector at 280°C. The initial oven temperature was set at 100°C, held for 1 minute, then gradually increased by 5°C/minute up to 280°C and held for 2 minutes, with the optimal flow rate condition selected at 1.2 mL/min. This method met all verification criteria and exhibited linearity in the 100–600 mg/L range with a high correlation coefficient of 0.9929, demonstrating its reliability and accuracy in determining caffeine content in coffee samples
Source: Engineering headway
DOI: 10.4028/p-xlxbq8
Authors: Salsabila Firdausi Zahra, Asnawati Asnawati, Zulfikar Zulfikar, Siswoyo Siswoyo, Tri Mulyono, Yeni Maulidah Muflihah
Institution: Universitas Jember
Language: English
Topic: Coffee research and impacts
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4412951160