FBXL3 serves as a suppressor of regenerative myogenesis.
Year: 2025
Type: article
Abstract: primary myoblasts. Gene set enrichment analysis (GSEA) revealed that FBXL3 deficiency enriches the gene set associated with striated muscle cell development, including MEF2C, a regulator of myogenin expression. Through a search in the ChEA3 database, TCF12 emerged as the downstream candidate gene regulated by FBXL3 to modulate MEF2C. ChIP-PCR assays confirmed the enrichment of TCF12 on MEF2C promoter at three consensus sites. Dual-luciferase reporter assay validated that TCF12 activates the MEF2C promoter. This comprehensive study underscores the crucial role of FBXL3 in satellite cell-mediated myogenic regeneration and provides insights into the intricate regulatory network involving TCF12 and MEF2C.
Source: PubMed
Authors: Wei He, Shiyuan Han, Yanming Wu, Min Chen, Ting Xue, Hua You, Ying Chang, Song-Bai Liu, Yi Sun, Yinjiang Tang, Xinghong Shi, Xingyu Han, Zixin Ma, Panting Qian, Sha Geng, Chaofan Wu, Yating Liang, Yangxin Li, Yan Xu, Yao-Hua Song
Institutions: Soochow University, State Key Laboratory of Radiation Medicine and Protection, Hainan Medical University, Suzhou Vocational Health College, Kunming Medical University, Fuwai Yunnan Cardiovascular Hospital, First Affiliated Hospital of Soochow University, Central South University, Second Xiangya Hospital of Central South University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4412957114