A new route for the determination of protein structure and function
Year: 2025
Type: article
Abstract: Understanding complex biological macromolecules, especially proteins, is vital for grasping their diverse chemical functions with direct impact on biology and pharmacology. Techniques such as x-ray crystallography and cryo-electron microscopy face limitations such as radiation damage and difficulties in crystallizing certain proteins. To address this, we present a strategic concept involving engineered protein scaffolds to create ordered arrays of proteins with controlled orientations, aiming at enhancing the signal at the detector. This innovative strategy opens avenues to solve the existing challenges for determining protein structures under physiological conditions. Moreover, it holds promise for studying conformational changes resulting from photoinduced changes, protein–drug, and/or protein–protein interactions. In the near future, our group will focus on showing the potential of the proposed strategy by combining molecular biology, protein self-assembly, and x-ray spectroscopy.
Source: AIP Advances
DOI: 10.1063/5.0214579
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,350
Awards: G121809054, G1266602394, G1446647517, G1855990336, G2262748287, G2290189609, G2316042581, G2358422808, G2660406951, G3480869486, G3518538256, G425668148, G4418367529, G4526231891, G4944594896, G5132640529, G5178665745, G5338730000, G5421974903, G598773271, G7084143925, G7685134749, G8920660035, G973521760
OpenAlex ID: W4413013260