Work

Steroid hormone-treated endometrial organoids enhance implantation of in vitro-produced porcine embryos

Year: 2025

Type: article

Abstract: IMPORTANCE: models that simulate this process are essential for studying early embryonic development. Endometrial epithelial organoids (EEOs) provide a promising platform to model these interactions. OBJECTIVE: This study aimed to verify the response of porcine EEOs to steroid hormones and assess how steroid hormones affect embryonic attachment in a culture medium containing EEOs. METHODS: environment, estradiol (E₂) was administered from day 3, followed by progesterone (P₄) treatment on day 5. On day 7, porcine EEOs were harvested using a cell recovery solution. We compared the untreated control group with the hormone-treated groups and measured the expression levels of E₂ and P₄ receptors. RESULTS: day and parthenogenetic embryos. CONCLUSION AND RELEVANCE: models to study implantation failure and infertility in reproductive medicine.

Source: Journal of Veterinary Science

DOI: 10.4142/jvs.25027