RNA Pol II-based regulations of chromosome folding
Year: 2025
Type: article
Abstract: The spatial organization of eukaryotic genomes and its dynamics are of functional importance for gene expression, DNA replication, and segregation. Structural maintenance of chromosome (SMC) complexes are essential instruments of chromosome folding, enabling long-distance intra-chromatid DNA loops. The interplay between these processes is complex. For instance, cohesin, in addition to tethering sister chromatids, dynamically regulates gene expression in mammals by promoting interactions between distal regulatory elements and promoters, whereas transcription itself affects genome folding in many ways. Here, we comprehensively dissect the relative contributions of transcription and cohesin complexes, as well as their interplay, to yeast S. cerevisiae genome organization. In particular, we show that transcription (1) is not a motor required to push cohesin during DNA loop expansion, (2) specifically induces the appearance of DNA loops independently of SMC complexes, and (3) interferes with cohesin-mediated DNA loop expansion during their establishment.
Source: Cell Genomics
Institutions: Centre National de la Recherche Scientifique, Université Toulouse III - Paul Sabatier, Institut Pasteur, Université Paris Cité, Department of Genomes & Genetics, Université de Toulouse, Centre de Biologie Intégrative de Toulouse, Unité de biologie Moléculaire, Cellulaire et du Développement, Université de Montpellier, Institut de Génétique Humaine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $8,900
Awards: G1255509621, G385027336, G5617386457, The dual life of RNA Polymerase I and snoRNP disordered KKE/D domains: from rRNA modification to nucleolar compaction, G6420661915, Investigating the functional architecture of microbial genomes with synthetic approaches, Functions of condensin: from a structural role to the control of genomic stability, Deciphering the determinants of cohesin-mediated genome folding, G8137860716
OpenAlex ID: W4413094661