Enhanced hematopoietic stem cell self-renewal and engraftment through human lung exosomal communication
Year: 2025
Type: article
Abstract: Hematopoietic stem cells (HSCs) are essential for sustaining the hematopoietic system throughout an individual's life. Within the HSC hierarchy, long-term HSCs are at the apex, maintaining the system by their unique capacity for self-renewal and differentiation into all blood cell lineages. Traditionally, it was believed that long-term HSC homeostasis is solely regulated within the bone marrow niche. However, our innovative single cell exosomal tracking analysis and in vivo transplantation studies revealed that lung cells play a significant role in this regulation through exosomal communication. Our in vivo primary and secondary engraftment studies demonstrate that exosomes derived from human primary small airway epithelial cells significantly enhance HSC engraftment. We developed a single-cell exosome tracking pipeline to track these exosomes and elucidated their effects on target cell populations. By integrating single-cell RNA sequencing, exosomal microRNA (miRNA) profiling, and proteomics, we have identified specific genes, miRNAs, and proteins that regulate HSC function, providing a comprehensive understanding of the molecular mechanisms involved in homeostasis. This insight suggests potential utility in modulating HSC function under experimental conditions, especially in conditions associated with low stem cell fitness including sickle cell disease and bone marrow failure.
Source: Molecular Therapy
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4413103308