Work

Protocol for recording and analyzing neuronal network activity ex vivo with multi-electrode arrays in mouse brain tissue

Year: 2025

Type: article

Abstract: Studying neuronal network activity is essential as brain functions arise from dynamic and coordinated interactions between neuronal populations. Here, we present a protocol for recording and analyzing network bursting activity in mouse hippocampal slices. We describe steps for preparing slices and inducing network activity. We then detail the recording procedure with multi-electrode arrays, followed by analysis. This protocol has potential applications for studying different types of network activity in brain tissues from various areas and species, from mice to humans. For complete details on the use and execution of this protocol, please refer to Dossi et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104019

Authors: Razmig Derounian, Nathalie Rouach, Elena Dossi

Institutions: Centre National de la Recherche Scientifique, Inserm, Collège de France, Université Paris Sciences et Lettres, Centre Interdisciplinaire de Recherche en Biologie

Language: English

Topic: Neural dynamics and brain function

Subfield: Cognitive Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Agence Nationale de la Recherche, Horizon 2020 Framework Programme, European Research Council, H2020 Marie Skłodowska-Curie Actions