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Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection

Year: 2025
Type: article
Abstract: Two-component system signalling is involved in several processes relevant to Clostridioides difficile virulence. We demonstrate that deletion of the orphan histidine kinase rgaS (R20291_0503), reduces detectable toxin in the supernatant of R20291 and impairs virulence in a mouse model of infection. These data suggest that RgaS regulates virulence capabilities in hypervirulent C. difficile . • The gene encoding histidine kinase rgaS (R20291_0503) was deleted in C. difficile R20291. • Cytotoxicity towards Vero cells was reduced for the rgaS mutant signifying a reduction in TcdA and TcdB in the supernatant. • Western blot analysis revealed a reduction in the amount of CDT in the supernatant of the rgaS mutant. • The mutant was less virulent in a mouse model of C. difficile infection, compared with the wildtype parental. • These data demonstrate the contribution of RgaS to C. difficile virulence.
Source: Anaerobe
DOI: 10.1016/j.anaerobe.2025.102988
Authors: Terry W. Bilverstone, Morgan E. Simpson, Nicholas Hagspiel, William A. Petri, Sarah A. Kuehne, Nigel P. Minton
Institutions: University of Nottingham, The Open University, Biotechnology and Biological Sciences Research Council, University of Virginia, Nottingham Trent University, Nottingham University Hospitals NHS Trust, Nottingham Biomedical Research Centre
Language: English
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $3,350
OpenAlex ID: W4413107012
FWCI:
Cites: 22
Cited by:
Related to: 10