Work

Rapid Detection of Staphylococcus aureus in Milk Samples by DNA Nanodendrimer-Based Fluorescent Biosensor

Year: 2025

Type: article

Abstract: Staphylococcus aureus is the primary pathogen responsible for mastitis in dairy cows and foodborne illnesses, posing a significant threat to public health and food safety. Here, we developed an enhanced sensor based on solid-phase separation using gold-magnetic nanoparticles (Au@Fe3O4) and signal amplification via dendritic DNA nanostructures. The substrate chain was specifically immobilized using thiol–gold coordination, and a three-dimensional dendritic structure was constructed through sequential hybridization of DNAzymes, L chains, and Y chains, resulting in a 2.8-fold increase in initial fluorescence intensity. Upon specific cleavage of the substrate chain at the rA site by S. aureus DNA, the complex dissociates, resulting in fluorescence intensity decay. The fluorescence intensity is negatively correlated with the concentration of Staphylococcus aureus. After optimization, the biosensor maintains a detection limit of 1 CFU/mL within 3 min, with a linear range extended to 1–107 CFU/mL (R2 = 0.998) and recovery rates of 85.6–102.1%, significantly enhancing resistance to matrix interference. This provides an innovative solution for rapid on-site detection of foodborne pathogens.

Source: Biosensors

DOI: 10.3390/bios15080527

Authors: Mukaddas Mijit, Dongxia Pan, Hui Wang, Chaoqun Sun, Liang Yang

Institutions: Chinese Academy of Agricultural Sciences, Institute of Animal Sciences, State Key Laboratory of Animal Nutrition, Guangxi University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,654